Fig. 3 | Scientific Reports

Fig. 3

From: Novel treatment strategy targeting interleukin-6 induced by cancer associated fibroblasts for peritoneal metastasis of gastric cancer

Fig. 3

CAFs promote the establishment and progression of PM of GC. (a) T3-2D cells (5 × 104 cells) alone or co-inoculated with MEF (1 × 105 cells) were inoculated into the abdominal cavity of C57BL/6J mice. Total numbers of PM were measured 14 days after tumor inoculation. Data are shown as the mean ± SD (n = 5). (b) Immunohistological analysis of α-SMA expression in PM. Tumor tissues were harvested 14 days after tumor inoculation. Scale bar, 100 μm. Areas of α-SMA expression in the peritoneal tumors were evaluated using an area index calculated by Image J. Data are shown as the mean ± SD (n = 3). Statistical significance was defined as p < 0.05 (*). (c) Intraperitoneal IL-6 concentration in peritoneal lavage was quantified by ELISA. Data are shown as the mean ± SD (n = 3). Statistical significance was defined as p < 0.05 (*).

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