Fig. 4

Anti-IL-6R Ab suppressed the progression of PM in CAF-mixed allograft model. T3-2D cells or T3-2D cells with MEF cells were intraperitoneally inoculated into C57BL/6J mice. Mice were treated with intraperitoneal administration of PBS or 500 µL of solution containing MR16-1 (200 µg/body) every 3 days for a total of 3 doses. (A) Schematic of the treatment schedule. Green arrows show the timing of treatment with PBS or MR16-1 and the vertical black line indicates sacrifice. (B) Orthotopic PM model established from T3-2D cells. The total number of tumors in the peritoneal cavity was measured 14 days after tumor inoculation. Data are shown as the mean ± SD (n = 5). There was no difference in total tumor numbers between the groups. (C) Orthotopic PM model established from T3-2D cells and MEF cells. The total number of tumors and tumor weight in the peritoneal cavity were measured 14 days after tumor inoculation. Data are shown as the mean ± SD (n = 5). Statistical significance was defined as p < 0.05 (*).