Table 1 Sequence information of ARMS-PCR primers used for amplifying FcγRIIA (rs1801274) and IL-10 (rs1800896) gene variants, along with their respective product sizes.
Primer | F/R | Sequence (5’→3’) | Length | Tm °C | GC % | Optimized Tm °C | Product size |
|---|---|---|---|---|---|---|---|
FcγRIIA rs1801274 | Forward inner | ACAACAGCCTGACTACCTATTACCTT | 26 | 60.7 | 42 | 63.7 | 316 bps |
Reverse inner | CATATTTGTGTCTTTCAGAATGGC | 26 | 60.7 | 42 | 269 bps | ||
Forward outer | GGAACATCCCAGAAATTCTCACA | 23 | 60.1 | 43 | 492 bps | ||
Reverse outer | GAACATCCCAGAAAATTCCACG | 22 | 59.4 | 45 | |||
IL-10 rs1800871 | Forward inner | GTACCCTTGTACAGGTGATGTCAC | 24 | 61.8 | 50 | 62.5 | 380 bps |
Reverse inner | GCAAACTGAGGCACAGAGCTA | 21 | 61.1 | 52 | 272 bps | ||
Forward outer | GGCTCCCCTTACCTTCTACAC | 21 | 63 | 57 | 607 bps | ||
Reserve outer | CAGAGACTGGCTTCCTACAGTACA | 24 | 62.3 | 50 |
