Fig. 4

Overview of Circulating Tumor DNA Process. Exceptional responders were identified from consecutive oligometastatic patients treated between 2014 and 2021. A clinically validated, personalized, tumor-informed 16-plex mPCR-NGS assay (SignateraTM, Natera Inc), was used to detect molecular residual disease (MRD). The Signatera assay is based on whole-exome sequencing of the tumor and a matched normal blood sample to detect and quantify ctDNA. Created with BioRender.com.