Fig. 1

Effects of 27 selected molecules at a single dose (50 µM) on in vitro 3CLpro enzymatic activity. Enzymatic activity was normalized to the control group (DMSO), which was set at 100. Data are presented as mean ± SEM (n = 2, with duplicates). The enzymatic activity rate was determined by dividing the fluorescence readings—recorded during the linear phase of product formation—by the corresponding time intervals. All other values were normalized to the control group, with its activity defined as 100.