Fig. 3
From: Induced protein expression in Leptospira spp. and its application to CRISPR/Cas9 mutant generation
Evaluation of conditional dnaK knockdown mutants inL. biflexa. (A) L. biflexa cells containing the plasmids pMaOri.Inducible dCas9 alone or with a sgRNA targeting the dnaK gene were seeded onto EMJH media containing spectinomycin without (-) or with ( +) 1 mM IPTG. Colony formation was monitored and recorded at different time points. (B) Colonies observed across four independent experiments. Number of colonies recovered in the absence or presence of IPTG was compared by t-test (*p < 0.05). Colonies from plates without IPTG (n = 3) were picked, cells released from the agar and inoculated in liquid HAN media containing spectinomycin at 105 cells/mL, in the absence or presence of IPTG, and growth was monitored daily at both 29 (C) and 37 °C (D).
