Table 1 Target and protospacer used in the sgRNA cassettes.
From: Induced protein expression in Leptospira spp. and its application to CRISPR/Cas9 mutant generation
Target | Protospacer (5′→3’) | Species |
|---|---|---|
dnaK (LEPBI_RS16560) coding strand | ACACGAGCCGCAATTTCTTG | L. biflexa |
ompL1 (LEPBI_RS04010) coding strand | CGAAACGTACGTTTTCTTTG | L. biflexa |
RNase P (LEPBI_RS18770) sense strand | TACTTGAGATTGCACATCGG | L. biflexa |
RNase P (LEPBI_RS18770) antisense strand | CGTTATGAGCCTGTCCCGCC | L. biflexa |
ompL1 (LIC_RS05025) coding strand | GCCGCCAGTAGTTCTATCGA | L. interrogans |
lipL21 (LIC_RS00055) | AACGTCTTTCGGATCGGATC | L. interrogans |
lipL41 (LIC_RS15260) | CTACGTTACGAATGGTTCCG | L. interrogans |
