Fig. 7

Stain effects of FOXA1 knockdown and overexpression on osteogenic differentiation of hBMSCs. (A-B) Knockdown of FOXA1 significantly enhanced hBMSC ALP activity (after 7 days of osteogenesis) and calcium deposits. (after 14 days of osteogenesis) in ALP and ARS staining. whereas overexpression of FOXA1 significantly reduced hBMSC ALP activity and calcium deposits in ALP and ARS staining. All data are means ± SDs (n = 3). * p < 0.05, **p < 0.01, and ***p < 0.001 versus the control group.