Fig. 3

(A) Alignment of the precursor peptides of leucocyclicin Q and leucocyclicin C showing the leader sequences are highlighted in yellow and a single threonine to alanine substitution in the structural peptide highlighted in blue. Identical amino acids (*) and conservative substitutions (:). (B) The nucleotide sequence of the leucocyclicin C propeptide. The cleavage site is highlighted by the dashed line between residues 2 and 3. RBS, putative ribosome binding site – 7 bp upstream of the start codon. *—stop codon.