Fig. 2 | Scientific Reports

Fig. 2

From: Comprehensive analysis of bacteriocins produced by clinical enterococcal isolates and their antibacterial activity against Enterococci including VRE

Fig. 2

Evaluation of the antibacterial activity of E. faecalis and E. faecium strains harboring bacteriocin genes via a soft-agar overlay assay. A soft-agar overlay assay was performed to evaluate the antibacterial activity against E. faecalis JARB-HU0683 and E. faecium JARB-HU0748. Overnight cultures of enterococcal strains (2.0 µl) were spotted onto TSA plates and incubated for 24 h at 37 °C. The indicator strain was added to 3.0 mL of prewarmed soft TSA agar medium, and the mixed medium was poured onto agar plates containing the previously spotted colonies and incubated aerobically overnight at 37 °C. The diameter of the growth-inhibitory zone surrounding the bacteriocin-producing bacteria was measured.

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