Fig. 5
C4 and C5 compounds induce intracellular ROS production in HCT16 and HT29 cells. (A) HCT116 and HT29 cells were cultured for a periodof 24 h in a medium enriched with 10% FBS. At 24 h, cells were treated with various concentrations viz. 50 µg/ml of C4 and 110 µg/ml of C5 respectively in HCT116 cells, whereas HT29 cells were treated with 110 µg/ml of C4 and 150 µg/ml of C5 respectively. At 24 h cells were stained with DCFH-DA and visualise uner Yokogawa CQ1 Confocal Imaging Cytometer to determine the ROS levels. The quantification of green signals was performed with the Image-J software. Using the mean of three investigations, fluorescent data were normalized with the positive control. (B) After treatment with C4 and C5 with varying concentrations (50 µg/ml of C4 and 110 µg/ml of C5 respectively in HCT116 cells, whereas HT29 cells were treated with 110 µg/ml of C4 and 150 µg/ml of C5 respectively) disrupts mitochondrial membrane permeability (MMP) for an additional 24 h.
