Fig. 6
(A) Colony formation assay was performed on proliferating HCT116 and HT29 cells treated with 50 µg/ml of C4 and 110 µg/ml of C5, whereas HT29 cells were treated with 110 µg/ml of C4 and 150 µg/ml of C5 respectively. The treatment of CRC cells with C4 and C5 exhibited significant reduction in colony forming potential of CRC cells. Whereas the treatment of HCT116 and HT29 with C4 led to 80.18% and 72.22% reduction respectively in percentage of colonies as compared to untreated cells. While treatment of HCT116 and HT29 with C5 led to 66.06% and 55.56% reduction respectively in percentage of colonies as compared to untreated cells. (B) Values are expressed as ± SD, *p ≤ 0.0001.
