Table 3 Details of primers employed in methylation-specific polymerase chain reaction.

From: Embryo vitrification impacts learning and spatial memory by altering the imprinting genes expression level in the mouse offspring’ hippocampus

Name

Nucleotide sequences (5′–3′)

mMeg3p

Forward

TTCCGGCCAATCACTTTAG

Reversed

TCAGGCAAAGGATGGCTAAC

mMeg3-Unmethylated

Forward

AACACATTACAACAAAATACACTAACA

mMeg3-Methylated

Forward

AACGCATTACAACAAAATACGCTAACG