Fig. 3 | Scientific Reports

Fig. 3

From: Knocking down the neuronal lactate transporter MCT2 in the arcuate nucleus of female rats increases food intake and body weight

Fig. 3

Robust inhibition of MCT2 expression in female rats transduced with AAVshMCT2 compared to control AAV. (A) Schematic of the experimental protocol showing the AAV constructs used: AAV-SYN-TdTOMATO as the control and AAV-shMCT2-SYN-TdTOMATO for MCT2 knockdown. Injection coordinates were as follows: anterior-posterior (AP): −3.14 mm, dorsal-ventral (DV): −9.8 mm, and left-right (LR): ± 0.30 mm. (B-E) Quantitative RT-PCR (qRT-PCR) analysis of MCT2, MCT1, MCT4, and HCAR1 mRNA levels in the hypothalami of rats transduced with control AAV (open bars) or AAVshMCT2 (closed bars) 2 weeks post-injection. Each experimental group processed in a different experiment was assigned a unique color, and three experiments were performed. (F) Western blot analysis showing MCT2 protein levels with actin as the loading control. (G) Semi-quantitative densitometric analysis of MCT2 protein levels normalized to actin, using three animals per condition. Data are presented as mean ± SEM. *p < 0.05; **p < 0.01 (unpaired t-test).

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