Fig. 4

AAVshMCT2 effectively targets the ARC and reduces MCT2 expression. (A-B2) Confocal microscopy analysis showing the localization of the tdTOMATO reporter (red) to confirm transduction of cells in the ARC. (C1-C2) Immunolocalization of MCT2 (green) to assess expression levels. (D1-D2) Immunolocalization of vimentin (white) to visualize tanycyte processes. (E1) Merged images illustrating the spatial relationship between tdTOMATO-expressing cells, MCT2, and tanycyte processes. (E2) High-magnification view of E1 highlighting the close association between tanycyte processes and MCT2-expressing neurons. White arrowheads indicate neurons transduced with tdTOMATO that lack MCT2 immunoreactivity. White arrows indicate neurons transduced with tdTOMATO that exhibit MCT2 immunoreactivity. Yellow arrowheads point to possible endothelial cells that are also vimentin-positive. The orange arrow indicates an astrocyte-like cell that is vimentin-negative but MCT2-positive. ME: median eminence; 3 V: third ventricle.