Fig. 5 | Scientific Reports

Fig. 5

From: Knocking down the neuronal lactate transporter MCT2 in the arcuate nucleus of female rats increases food intake and body weight

Fig. 5

AAVshMCT2 reduces MCT2 immunoreactivity in the ARC. Immunohistochemistry analysis of MCT2 (green) with DAPI (blue) as a nuclear marker in rats transduced with (A-C) control AAV and (D-F) AAVshMCT2. (C and F) 3-D views reconstructed from 15 confocal z-planes spanning a total of 10 μm, demonstrating the reduction of MCT2 signal in AAVshMCT2-injected rats compared to control. The 3D projection was generated using Zen Software Version 8.0, which includes image acquisition, processing, and a module for 3D visualization and analysis. The images were obtained using a ZEISS LSM780 confocal laser scanning microscope (Carl Zeiss Microscopy GmbH, Germany). More information about the software can be found at: https://www.micro-shop.zeiss.com/es/uy/softwarefinder/software-categories/zenblack/.

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