Fig. 3
Characterization of COSCC cell lines. (A) Cell lysates were resolved via SDS-Page, and blotted for murine cyclophilin A (CyPA), phospho-ERK (p.ERK), or vinculin (loading control). 4T1 and E0741 are murine breast cancer cell lines, which show a signal for the CyPA protein, while U87 is a human glioblastoma cell line which is negative for the protein. Three of the novel cell lines show signal for murine protein. All of the novel cell lines have some phospho-ERK signal, suggesting intact RAS signaling. (B) For each cell line, 1000 cells were plated into a 10 cm plate, and allowed to grow for 10–20 days. The plates were stained with crystal violet to visualize colony formation.
