Fig. 5
Dose curves for assorted drugs vs. COSCC cell line Co-F-1236. Cells were plated at low density (2000 cells per well) in 96 well plates and treated for 6 days with the indicated amounts of each inhibitor (A) docetaxel, (B) 5-fluorouracil, (C) cetuximab, (D) carmustine, (E) temozolomide, (F) trametinib, (G) dabrafenib). The cells were then treated with PrestoBlue reagent, and cell viability was determined. %Inhibition was determined by comparison to no-drug controls (representing max growth) and cell-free lanes (representing 100% cell death). Each data point represents the average measurement from three samples, and the error bars represent the standard deviation from the mean. The lowest-concentration data point for each graph is a no-drug control and is given a non-zero value to be visible on the semi-log plot.
