Fig. 4 | Scientific Reports

Fig. 4

From: Separation of pups from their mother mice enhances odor associative learning at the late lactation stage

Fig. 4

An odor preference is formed by odor experience with siblings during maternal separation. (A) Locomotion of oil-nip-conditioned (n = 13) and EG-nip-conditioned pups (n = 15) conditioned during P12-19 was traced in an odor preference test for EG at P19 (left). EG-nip-conditioned pups showed no odor preference (right). Welch’s unpaired t-test. (B) Preference index for EG in oil-dam-unpaired (Oil-DUP, n = 12), EG-dam-unpaired (EG-DUP, n = 14), EG-dam-paired conditionings (EG-DP, n = 8), EG-simple exposure-conditioned (EG-SE, n = 13) and EG-dam/sibling-unpairing-conditioned pups (4EG-DSUP, n = 14). Pups in EG-DUP and EG-DP conditionings displayed significant preference while those in EG-SE and EG-DSUP conditionings displayed no significant preference. Tukey’s multiple comparison test: **, p < 0.01; ***, p < 0.001. OST (A, B): $, p < 0.05; $$$$, p < 0.0001.

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