Fig. 5 | Scientific Reports

Fig. 5

From: Aloe polysaccharide promotes keratinocyte proliferation, migration, and differentiation by upregulating the EGFR/PKC-dependent signaling pathways

Fig. 5

ACE activates EGFR, AKT, and PKC as PMA. (A) HaCaT cells were treated with PMA (100 nM) or ACE (3 mg/ml) for indicated times to determine EGFR, AKT, and PKC phosphorylations. (B) HaCaT cells were pretreated with TH1020 (2 µM) or osimertinib (1 µM) for 30 min and followed by ACE treatment. The effects of TH1020 and osimertinib on ACE-induced phosphorylation of AKT and PKC were determined. (C) NHEK cells were pretreated with osimertinib or DMSO (vehicle) for 30 min, then treated with ACE (3 mg/ml) for 1 and 3 h. The relative protein expression level was normalized by β-actin, and the phosphor-protein expression level was shown with the normalization by their endogenous protein level. The line charts representing quantifications of protein expression levels in each panel were shown in Figure S6.

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