Fig. 5
From: Optimized laboratory techniques for assessing the quality of pre-stripped DMEK grafts
Tips for performing IF after viability assessment. Double IF of NCAM (FITC filter) and ZO-1 (Cy3 filter) was conducted on a DMEK graft that had already undergone viability testing and additional TB staining. (A) IF was performed immediately after HEC staining. (B) IF was performed on methanol-fixed tissue that was rinsed in PBS for 24 h following the HEC staining step. (C) IF was performed on methanol-fixed tissue that was rinsed in PBS for 24 h following the HC staining (without Ethidium).
