Fig. 5
CID 662896 and CID 5308648 correct LMNB1-induced associated nuclear abnormalities. A. TRE-FLAG-LMNB1 MEF were exposed to doxycycline for three days, leading to elevated LMNB1 levels and characteristic nuclear abnormalities (+ DOX vs. -DOX) (cf. Nmezi, 2020). B. Images of DOX stimulated cells in the presence of vehicle (DMSO, + DOX), CID 662896, or CID 5308648 were acquired on an Opera Phenix Plus high-content reader using a 20X objective and analyzed for nuclear expression of LMNB1 and associated nuclear abnormalities by a machine-learning texture (SER texture) algorithm in Harmony 5.1. Left panels, original fluorescence micrographs; right panels, images with algorithm applied. Green nuclei, normal; red nuclei, abnormal). C. Quantification of nuclear abnormalities. Each data point is the average of four independent biological replicates ± SD. EC50 values were calculated for each replicate by a four-parameter logistic equation and averaged.
