Fig. 6 | Scientific Reports

Fig. 6

From: A high throughput, high content screen for non-toxic small molecules that reduce levels of the nuclear lamina protein, Lamin B1

Fig. 6

Activity of prioritized hits in ADLD patient cells. Cultured fibroblasts from ADLD patients were treated for 48 h with compounds. Lysates were immunoblotted with anti-LMNB1 and anti-beta actin antibodies. A and B. Representative Western blot of ADLD cells treated with CID 662896 or CID 5308648, respectively. Markers on the left indicate molecular weights in kDa. C and D. Quantification of CID 662896 and CID 5308648 response. LMNB1 / actin intensity ratios were normalized to DMSO controls. Data in C. and D. show mean ± SD of the indicated numbers of independent biological repeats. P-values were computed by one-way ANOVA with Holm-Šídák's multiple comparisons test.

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