Fig. 13

NNMT expression in relation to clinical outcomes in patients. (a) Immunofluorescence staining of NNMT expression in tumor (T) and adjacent non-tumor (AT). Scale bars represent 50 μm. (b) Violin plot showing NNMT expression in HCC tissues with or without macrovascular invasion. (c) NNMT expression in HCC tissues with or without relapse. (d) NNMT expression in relation to OS. (e) mRNA expression of NNMT in HepG2 cells transfected with control (NC) or NNMT overexpression (OE-NNMT) plasmid. (f) Cell viability measured by CCK-8 assay at 24, 48, and 72 h. (g) Flow cytometry analysis of cytotoxicity in OE-NNMT and NC HepG2 cells. h: Flow cytometry analysis of cell cycle distribution in OE-NNMT and NC HepG2 cells.