Fig. 5 | Scientific Reports

Fig. 5

From: Carbon ion stimulation therapy reverses iron deposits and microglia driven neuroinflammation and induces cognitive improvement in an Alzheimer’s disease mouse model

Fig. 5

Results of Western blot analysis (A) used to measure cytokine release by anti-inflammatory microglia, TGF-β (B), TNF-α (C), and IL-1β (D) to determine the proinflammatory response, suggesting that the microglial phenotype changed from proinflammatory to anti-inflammatory one month after CIS treatment. Turnbull staining of microglial BV2 cells treated with Aβ-Fe3O4 fibrils revealed depletion of Aβ-Fe3O4 fibrils within BV2 cells after CIS treatment (E), suggesting that iron is released in BV2 cells upon disruption of Aβ-Fe3O4 fibrils or subsequent microglial ferroptosis.

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