Fig. 3

Immunoproteasome expression is induced in CGRPβ knockout (KO) mice 3 days after consumption of dextran sulphate sodium (DSS). (a) Images of haematoxylin and eosin (HE)-stained colon tissue sections from wild-type (WT), CGRPα KO, and CGRPβ KO mice with DSS treatment. The black scale bar represents 100 μm. (b) Pathology scores were calculated based on HE-stained images performed on colon tissue after 3 days of DSS treatment. For histological analysis, four WT mice, three CGRPα KO mice and three CGRPβ KO mice were used. (c) Results of the principal component analysis based on the colon proteome analysis of samples prepared after 3 days of DSS consumption. For proteomic analysis, four WT mice, three CGRPα KO mice and four CGRPβ KO mice were used. (d) Volcano plot showing comparison of protein levels between WT and CGRPβ KO mice. (e) GSEA of protein expression between WT and CGRPβ KO mice, with positive normalized enrichment score terms indicating higher expression in CGRPβ KO mice and negative terms indicating higher expression in WT mice. (f) Venn diagram of proteins whose expression was up-regulated in CGRPα KO and CGRPβ KO mice compared with that in WT mice in the proteome analysis. Proteins whose expression was specifically up-regulated in CGRPβ KO mice were analysed. (g) Results of STRING analysis: STRING focused on protein–protein interactions, and reactome enrichment analysis was applied to colour the protein nodes belonging to each term. (h) Western blot analysis of the expression of immunoproteasome subunits PSMB8, PSMB9, and PSMB10 in the colon samples after 3 days of DSS consumption.