Table 1 Time table of the gradient elution used for chromatographic separation of TAM and TTD.
Time (min) | solvent A (%) (Acetonitrile) | solvent B (%) (Water) | solvent C (%) (Phosphate buffer pH 3) | Flow rate mL/min |
|---|---|---|---|---|
Initial | 40 | 60 | 0 | 1.0 |
1 | 40 | 60 | 0 | 1.0 |
5.5 | 50 | 0 | 50 | 1.0 |
9 | 80 | 0 | 20 | 1.0 |
10 | 40 | 60 | 0 | 1.0 |
