Fig. 2 | Scientific Reports

Fig. 2

From: Engineered PD-L1 co-expression in PD-1 knockout and MAGE-C2-targeting TCR-T cells augments the cytotoxic efficacy toward target cancer cells

Fig. 2

Exogenous expression of PD-L1 in MC2-TCR-T cells inhibited TCR expression and its functions. A Flow dot plot showing PD-L1 on T cells after CD274 gene transduction. B PDL1-MC2-TCR-T cells and MC2-TCR-T cells were compared for their affinity for the MC2336−344-HLA-A*02:01 tetramer, as detected by flow cytometry. C, D Representative flow dot plots and histogram showing IFN-γ production in PDL1-MC2-TCR-T and MC2-TCR-T cells stimulated with MC2-T2 cells via ICS staining. E, F Representative flow dot plots and histogram showing IFN-γ production in PDL1-MC2-TCR-T and MC2-TCR-T cells stimulated with MC2-A02-K562PD1+ cells via ICS staining. G TNF-α secretion by PDL1-MC2-TCR-T and MC2-TCR-T cells after stimulation by coculture with MC2-T2 (left) and MC2-A02-K562PD1+ (right) cells, respectively. Control peptide (HPV16-E6)-loaded T2 cells (C-T2) and empty HLA-A*02:01-transducted K562 cells (A02-K562) served as controls. The results are from three individual replicates. One-way ANOVA was used for statistical analysis. Different lowercase letters (a, b, c…) above the bars designate statistically significant differences (P < 0.05).

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