Fig. 3
Exogenous PD-L1 expression and endogenous PD-1 knockout enhanced MC2-TCR-T cytokine secretion. A Representative flow cytometry dot plots illustrating PD-1 expression in two TCR-T models before and after PD-1 knockout, detected using an anti-PD-1 antibody. The PD-1+ population is highlighted within the squared regions. B Histogram quantifying the percentage of PD-1+ cells from panel A. C Representative flow cytometry dot plots showing dual detection of MC2-TCR and PD-L1 expression in different TCR-T models using the MC2336−344-HLA-A*02:01 tetramer and an anti-PD-L1 antibody. D Histograms depicting the percentages of tetramer-bound cells post-transduction (left) and the proportion of PD-L1-expressing cells within the tetramer-bound population (right). E IFN-γ production in various TCR-T cells stimulated with MC2-A02-K562PD1+, measured via intracellular cytokine staining (ICS). F Histogram showing the quantitative analysis of ICS-positive cells. G TNF-α secretion, assessed using an ELISA kit, in various TCR-T cells stimulated with MC2-A02-K562PD1+. All results represent data from three independent replicates. Statistical analysis was performed using one-way ANOVA. Different lowercase letters ( a, b, c…) above the bars designate statistically significant differences (P < 0.05).
