Fig. 11 | Scientific Reports

Fig. 11

From: ABCE1 facilitates tumour progression via aerobic glycolysis and inhibits cell death in human colorectal cancer cells through the p53 signalling pathway

Fig. 11

Irinotecan inhibits cell growth and aerobic glycolysis via p53 signalling in HCT-116 cells. (A) Gene Set Enrichment Analysis (GSEA) plot illustrate the involvement of p53 signalling pathway from COAD dataset (GSE33113). (B) HCT-116 cells were subjected to treat with irinotecan with and without nut-3a cell viability was measured. (C and D) Glucose uptake and lactose production were measured by treating irinotecan and combing with nut-3a in HCT-116 cells. (E and F) ROS activity was also measured by evaluating the cellular SOD and CAT by treating irinotecan and nut-3a alone and in combined in HCT-116 cells. (G) cell cycle analysis by flow cytometry was measured by treating irinotecan with and without nut-3a in HCT-116 cells. (H) Cell death by Annexin V-FITC/PI double staining was measured by treatment of irinotecan and nut-3a in HCT-116 cells. (I) Gene expression of p53, p21, BAX, BCl2, LDHA, and GLUT2 was examined by RT-PCR by treating irinotecan and nut-3a alone and in combined in HCT-116 cells. Statistical significance: *p < 0.05, **p < 0.001, and ***p < 0.0001.

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