Fig. 3

The mRNA expression levels of ABCE1 in COAD datasets. (A, B) The GSEA plot from GSE33113 datasets indicates the upregulation of ABCE1 from KEGG pathways denotes highly involved in Pathways in cancer (NES-1.12) and colorectal cancer (NES-1.22). The green line indicates the NES and red color to blue color mentions the tumour (positively correlated) and normal (negatively correlated) from COAD datasets. The bar code represents each gene involved in expression levels in COAD datasets. (C-D) Bioinformatic analysis mentioned the exon, Junction, and Isoform expression level of ABCE1 from HGNC datasets among various cancers, in colon, ABCE1 has highlighted. (F) mRNA expression levels of ABCE1 in COAD datasets from UALCAN in various stages (Stage I (n = 45), II (n = 110), III (n = 80), and IV (n = 39) compared to normal (n = 41) colon tissues from TCGA clinical samples. Moreover, (G) GEPIA datasets also shows high elevation of advanced stage mRNA expression (F value = 2.95) compared to an early stage from COAD datasets. (H) ABCE1 expression levels in different age groups (21–100) compared with normal (n = 41) colon tissues were also visualized using UALCAN database. (I) To find the prognostic value of ABCE1 in COAD datasets, nodal metastasis (N0 (n = 166); N1 (n = 70); N2 (n = 47)) for ABCE1 from TCGA datasets. (J) The overall survival of ABCE1 from COAD datasets were retrieved from GEPIA database and visualized by Kaplan-Meier plotter. The red color mentioned the high ABCE1 and blue color denotes the low ABCE1 expression. (K) The protein expression levels of ABCE1 from CPTAC samples of COAD datasets shows high expression level (n = 97) compared with normal (n = 100). (L) The protein expression levels of ABCE1 were visualized by immunoblot in normal colon mucosa, NCM-460 and colorectal cancer cells, HCT-116 and HT-29. β-actin as an internal control. (M) ABCE1 mRNA expression levels between control (n = 41) and tumour samples (n = 286) were monitored using TCGA-COAD datasets. (N) The gene expression of ABCE1 by qRT-PCR was examined in HCT-116 and HT-29 cells. Statistical significance: *p < 0.05, **p < 0.001, and ***p < 0.0001. Uncropped western blot images corresponding to Fig. 2 can be found in Supplementary Fig. 4a. GSEA, Gene Set Enrichment Analysis; KEGG, Kyoto Encyclopedia of Genes and Genomes; NES, Normalized Enrichment Score; HGNC, HUGO Gene Nomenclature Committee; CPTAC, Clinical Proteomic Tumour Analysis Consortium; RT-PCR, Reverse Transcription Polymerase Chain Reaction.