Fig. 6
(A) The effect of mir320a-3p restoration on apoptosis of GC cell lines (AGS, MKN-45, and KATO III) after restoration. The cells were transfected with IC-50 concentration to characterize apoptosis rates by the Annexin-V/PI method. According to the graphs, the Q1 area displayed cells with necrosis, Q2, Q3, and Q4 areas represented the late, early apoptotic, and viable cells, respectively. As the figure shows, the percentage of apoptosis increased in transfected groups compared to control cells. (B) DAPI staining determined the induction of apoptosis in AGS, MKN-45, and KATO III cell lines, respectively, compared to control and negative control groups. Apoptotic cells (Arrows indicate apoptotic cells)were identified based on nuclear condensation, fragmentation, and morphological features such as cell shrinkage and membrane blebbing. Statistical significance was assessed using an unpaired t-test (**P value < 0.01 and ***P value < 0.001).
