Fig. 3 | Scientific Reports

Fig. 3

From: Exploring a patient-specific in vitro pipeline for stratification and drug response prediction of microglia-based therapeutics

Fig. 3

Separation of individual cytokine profiles based on disease status in 2D and 3D models. Heatmaps of cytokine responses (mRNA and secretion) in HC, MCI and AD individuals with hierarchical clustering of donor profiles (n = 12) and cytokine responses (total n = 19, which includes n = 12 cytokines for secretion analyses and n = 7 cytokines for mRNA analyses) at (A) baseline, and following treatment with (B) minocycline and (C) IC14 in 2D and 3D models. Heatmap values were normalised to LPS (minocycline) or LPS with isotype (IC14), log-transformed and scaled. PCA analyses, presented as plots below each heatmap, included all normalised cytokine response values (n = 19) from each donor. Black circles in A and B include individuals with similar profiles.

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