Fig. 3

(A) Relative abundance plot depicting the distribution of virulence factor-associated genes across different samples. A total of 445 types of virulence factor-associated genes were identified, with immune modulation being most abundant in Fish Intestine and Sediment. The effector delivery system was highest in Chicken Gut and Sediment, while biofilm formation was predominant in Feed. Additionally, motility and adherence mechanisms were notable in Droppings and Chicken Gut, highlighting the diverse virulence profiles across samples. (B) Bar plot illustrating the abundance of virulence factor-associated genes across different sample types. Significant differences in virulence factor abundance were observed among the samples, with Sediment exhibiting the highest count compared to Feed, Chicken Gut, Fish Intestine, and Droppings. Droppings also showed significant differences when compared to Feed and Chicken Gut, while Chicken Gut had notable differences compared to Fish Intestine. The number of virulence factors identified in each sample varied, highlighting the diverse virulence profiles present.