Table 1 Limit of detection (LOD) tests of malaria Pan qPCR, malaria Pan/Pf/Pv/IC LAMP-MS assay, and BinaxNOWâ„¢ malaria test (P/N) using 10-fold serial diluted P. falciparum samples*.

From: Development of a rapid malaria LAMP-MS assay for diagnosis of malaria infections

Assays

DNA extraction

Target

P. falciparum parasites/µl

1.1 × 105

1.1 × 104

1.1 × 103

1.1 × 102

1.1 × 101

1.1 × 100

1.1 × 10− 1

DW

Malaria Pan qPCR (Ct)

Chelex-100

Pan

22.28 ± 0.05

24.88 ± 0.15

28.07 ± 0.05

31.49 ± 0.03

33.96 ± 0.11

N/A

N/A

N/A

Qiagen

Pan

17.25 ± 0.07

20.94 ± 0.07

24.35 ± 0.02

28.12 ± 0.13

31.81 ± 0.41

N/A

N/A

N/A

Malaria Pan LAMP-MS (P/N)

Chelex-100

Pan

P

P

P

P

N

N

N

N

Pf

P

P

P

P

N

N

N

N

Pv

N

N

N

N

N

N

N

N

IC

P

P

P

N

N

N

N

N

Qiagen

Pan

P

P

P

P

N

N

N

N

Pf

P

P

P

P

N

N

N

N

Pv

N

N

N

N

N

N

N

N

IC

P

P

P

N

N

N

N

N

BinaxNOWâ„¢ Malaria TEST (P/N)

 

Pan

P

P

P

N

N

N

N

N

Pf

P

P

P

N

N

N

N

N

  1. *qPCR and LAMP-MS were performed on DNA extracted by Chelex-100/boiling method and QIAamp DNA Blood Mini Kit, while BinaxNOWâ„¢ Malaria Test was conducted using direct blood sample dilutions. Ct: Cycle Threshold; P/N: Positive (P) or Negative (N); N/A: Not Applicable.
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