Table 2 Limit of detection (LOD) tests of malaria Pan qPCR, malaria Pan/Pf/Pv/IC LAMP-MS assay, and BinaxNOWâ„¢ malaria test (P/N) using 10-fold serial diluted P. vivax samples*.

From: Development of a rapid malaria LAMP-MS assay for diagnosis of malaria infections

Assays

DNA extraction

Target

P. vivax parasites/µl

2 × 104

2 × 103

2 × 101

2 × 101

2 × 100

2 × 10− 1

1.1 × 10− 2

DW

Malaria Pan qPCR (Ct)

Chelex-100

Pan

25.46 ± 0.17

27.00 ± 0.15

30.13 ± 0.10

34.56 ± 0.41

N/A

N/A

N/A

N/A

Qiagen

Pan

19.39 ± 0.55

22.68 ± 0.33

25.85 ± 0.04

29.93 ± 0.07

34.26 ± 0.56

N/A

N/A

N/A

Malaria Pan LAMP-MS (P/N)

Chelex-100

Pan

P

P

P

N

N

N

N

N

Pf

N

N

N

N

N

N

N

N

Pv

P

P

P

N

N

N

N

N

IC

P

P

P

N

N

N

N

N

Qiagen

Pan

P

P

P

N

N

N

N

N

Pf

N

N

N

N

N

N

N

N

Pv

P

P

P

N

N

N

N

N

IC

P

P

P

N

N

N

N

N

BinaxNOWâ„¢ Malaria TEST (P/N)

 

Pan

P

P

N

N

N

N

N

N

Pf

N

N

N

N

N

N

N

N

  1. *qPCR and LAMP-MS were performed on DNA extracted by Chelex-100/boiling method and QIAamp DNA Blood Mini Kit, while BinaxNOWâ„¢ Malaria Test was conducted using direct blood sample dilutions. Ct: Cycle Threshold; P/N: Positive (P) or Negative (N); N/A: Not Applicable.
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