Fig. 2

PCOS induces NAFLD-like biochemical and histological alterations. The figure shows changes in (A) body weight after PCOS induction, biochemical profile of fasted animals (A) plasma glucose, (C) triglycerides, (D) total cholesterol, (E) LDL-cholesterol, (F) HDL-cholesterol, (G) IPGTT, (H) AUC of IPGTT, (I) serum insulin, (J) HOMA-IR, (K) ALT, (L) AST, (M) ALP, (N) liver index (%), (O) serum adiponectin, (P) immunoblots of liver adiponectin and actin (n = 3), (Q) densitometric analysis of adiponectin immunoblot normalized to actin, (R) mRNA expression of adiponectin in the liver normalized to actin, (S) gross morphology of control and PCOS liver, (T) representative photomicrographs of H&E stained liver sections (U) representative photomicrographs of oil red O stained sections of liver and (V) representative photomicrographs of transmission electronic microscopy (TEM) of liver sections; black arrows indicate the hepatocellular ballooning or lipid droplets; (W) Ballooning area (%). Two-way ANOVA plus Bonferroni’s post hoc test was performed in (A), and unpaired two-tailed t-tests were performed in (B-F), (H-O), and (Q-R-W). All blots were cut prior to hybridization. Data are presented as mean ± S.D; (n = 5); *p < 0.05, **p < 0.01 and ***p < 0.001, (*) vs. Control group.