Fig. 5

AdipoRon restores hepatic clock gene expression in PCOS-induced NAFLD. The figure shows (A) immunoblots of BMAL1, CLOCK, CRY2, PER3, REVerbα, RORα and β-actin, (B) densitometric analysis of BMAL1 normalized to β-actin, (C) densitometric analysis of CLOCK normalized to β-actin, (D) densitometric analysis of CRY2 normalized to β-actin, (E) densitometric analysis of PER3 normalized to β-actin, (F) densitometric analysis of REVerbα normalized to β-actin, (G) densitometric analysis of RORα normalized to β-actin, and (H-M) mRNA levels of Bmal1, Clock, Cry2, Per3, Reverba and Rora normalized to β-actin. One-way ANOVA plus Tukey’s post hoc tests were performed in (B-M). All blots were cut prior to hybridization. Data are presented as mean ± SD; (n = 3); *or#p < 0.05, **or##p < 0.01 and ***or###p < 0.001, (*) vs. Control group and (#) vs. PCOS group.