Fig. 2

Mvh gene in knock-in allele is defective. (a) Histological analyses of Vasa^wt/− and Vasa^−/Tk30 mouse testes (left) and epididymis (right). Scale bars: 100 µm (left) and 200 µm (right). (b) PAS-hematoxylin staining of Vasa^wt/− and Vasa^−/Tk30 mouse testis. Spermatogenesis stages are shown. Scale bars: 10 µm. (c) qRT-PCR analysis of Vasa^wt/− and Vasa^−/Tk30 mouse testis. Error bars represent the standard error of biological replicates (n = 2). The expression levels were normalized to GAPDH expression level.