Fig. 2

(A) Limit of detection analysis of rt-LAMP for the mpt64 gene A. l to 9 serially diluted pL-mpt64 DNA with a concentration of 1.91 × 108, 1.91 × 107, 1.91 × 106, 1.91 × 105, 1.91 × 104, 1.91 × 103, 1.91 × 102, 1.91 × 101, 1.91 × 100 copies/µl and 10 : No Template Control. (B) The standard curve of the rt–LAMP assay shows a linear correlation of concentration DNA with amplification time R2 = 0.97. n = 3.