Fig. 1

Summary of FISH results in V. acanthurus and V. citrinus individuals with normal sex chromosomes and individuals with enlarged microchromosomes. The W chromosome is homologous with the telomeric region of the long arm on chromosome 1 as a balanced duplication and with the unpaired enlarged microchromosome as an unbalanced segmental duplication. In V. citrinus the Z chromosome exhibited several segmental duplications with the centromere of a number of microchromosomes. ZW sex chromosomes within yellow box show hybridisation patterns of Z and W probes observed in 3 females of V. citrinus with homomorphic sex chromosomes. ZW sex chromosomes within white box show hybridisation patterns of Z and W probes observed in heteromorphic Z and W chromosome as well as enlarged unpaired microchromosomes from 1 female V. citrinus. EM is an enlarged and unpaired microchromosome first described by Matsubara et al.46; n indicates number of individuals screened.