Fig. 3 | Scientific Reports

Fig. 3

From: Engineering IL-10 and rapamycin to bind collagen leads to improved anti fibrotic efficacy in lung and kidney fibrosis

Fig. 3

A3-IL-10 rescues the fibrotic damage in mouse model of lung fibrosis. Lungs insulted with 75 µg bleomycin; 1 week following instillation mice were left untreated (A, E) or injected i.v. with a total of 40 µg (or molar equivalent) in 4 injections of 10 µg on days 7, 10, 14 and 17 post bleomycin insult with either (B, F) IL-10, (C, G) A3-IL-10, (D, H) A3-SA-IL-10. Insets are pictured and the size bar is 2000 μm. (I) Mouse body weights after bleomycin insult and treatment. (J) Collagen content from the right, multi-lobed lung assessed by hydroxyproline assay. (K) Blinded Ashcroft scoring. N = 8. * = statistical significance of P < 0.05, significance vs. fibrotic lungs calculated by 2-way ANOVA (with Fisher’s LSD post-test). Comparison between IL-10 and A3-IL-10 is Student’s t-test. Error bars are SEM.

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