Fig. 4
Rapamycin and rapamycin-CBP-micelle treatments rescue the fibrotic damage from UUO insult to mouse kidneys. The descending ureter of the left kidney was surgically ligated, and 3 mg/kg (or molar equivalent) rapamycin and rapamycin-CBP-micelles was injected i.v. at 7, 9, 11, and 13 days post UUO ligation. Kidneys were resected 14 post UUO insult, mounted, and assessed via immunohistochemistry for collagen I. Representative images for (A) uninjured kidneys, (B) fibrotic kidneys, (C) and fibrotic kidneys treated with (C) rapamycin, and (D) rapamycin-CBP-micelles. (E) The amount of positive IHC staining was compared to the overall amount of kidney tissue, per image. N = 6. * = statistical significance of P < 0.05, one-way ANOVA vs. fibrosis control, Welch’s correction. Comparison between rapamycin and rapamycin-CBP-micelles is Student’s t-test. Error bars are SEM.
