Fig. 3 | Scientific Reports

Fig. 3

From: Viral mediated α-synuclein overexpression results in greater transgene levels and α-synuclein overload in mice bearing kinase dead mutation of LRRK2

Fig. 3

AAV-h-A53Tα-syn injection results in greater pSer129 α-syn levels in the SN of LRRK2 KD mice compared to WT mice. Immunohistochemistry analysis of pSer129 α-syn and total α-syn staining in the SNpc of 12-month-old WT and LRRK2 KD mice 3 months after AAV-h-A53Tα-syn or AAV-GFP injection. Representative images and quantification of nigral pSer129 α-syn (A) and total α-syn (B) staining. Data are expressed as immunopositive surface of the structure of interest ± SEM of: panel (A), n = 8 mice per group; panel (B), n = 9 (WT AAV-h-A53Tα-syn, KD AAV-GFP) or n = 10 (WT AAV-GFP, KD AAV-h-A53Tα-syn) mice per group. **p < 0.01 (Student’s t test two-tailed for unpaired data or two-way ANOVA followed by the Tukey’s test for multiple comparisons).

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