Fig. 3
From: Quantitative detection of β-glucans in Cordyceps species using a validated Congo red assay

Temperature has a significant effect on the extraction efficiency of β-glucan, and the best extraction effect was achieved at a temperature of 65 °C (left image: β-glucan standard; right image: YBC). The results were represented as the mean value of β-glucan content ± standard deviation (SD) in three parallel experiments (n = 3).