Fig. 5
From: In vitro enzymatic and cell culture assays for SARS-CoV-2 main protease interaction with ambenonium
Cytotoxicity assay for Vero and HEK cells using Alamar blue method. Cells were incubated for 24 h, 48 h and 72 h with ambenonium in different concentrations. The gray area in the graphics corresponds to the cellular viability, ranging from 80 to 100%. The blue line shows the cellular viability in the presence of ambenonium, the orange dashed line shows the viability in the presence of DMSO, and, in red, the viability in the presence of Triton. The data are representative of at least seven internal replicates, with STD and mean. The data were subjected to a Kruskal–Wallis test followed by Dunn’s Test using GraphPad Prism version 5.03. The asterisks mean statistical difference with p < 0.05 in relation to ambenonium. * means p < 0.05, ** means p < 0.01 and *** means p < 0.001.
