Fig. 2

(a) Schematic representation of T-cells trapped in PDMS microwells, measured by tipless AFM cantilever. (b) Cells positioned in traps: Cells are labeled with Alexa Fluor. (All cells are labeled, and the trapped cells are highlighted using the software). (c) Typical indentation/ retraction curve produced by deflection of AFM cantilever vs vertical position of AFM stage (and the equation used to extract the young’s modulus E from the indentation curve). (d) Schematic representation of the ML process applied for the prediction and classification based on the AFM data.