Fig. 4

H37Rv infection promotes macrophage autophagy and progressively upregulates miR-30c-1-3p and suppresses ATG4B and ATG9B expression. (A–C) RT-PCR was performed to detect the expression of miR-30c-1-3p and its target genes at different infection time nodes. The expression of miR-30c-1-3p exhibited a gradual increase following infection, reaching a peak at 12 h post-infection, and the expression of ATG4B and ATG9B exhibited an opposite trend. (D) Western Blot demonstrated that H37Rv infection for 2 h promoted macrophage autophagy. H37Rv increased LC3-II protein levels after infection of macrophages for 2 h. The application of Baf-A1 (100 nM) resulted in the accumulation of LC3-II. The LC3 bands were quantified relative to β-actin. All data above represent the means ± SD of at least three independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not statistically different. Multiple comparisons between groups were made using the Waller-Duncan method. Different letters represent P < 0.05 and the presence of any same letter represent P > 0.05.