Fig. 2 | Scientific Reports

Fig. 2

From: Author Correction: Hyaluronic acid 35 normalizes TLR4 signaling in Kupffer cells from ethanol-fed rats via regulation of microRNA291b and its target Tollip

Fig. 2

siRNA knockdown of HA receptors in Kupffer cells from pair- and ethanol-fed rats. Wistar rats were allowed free access to a Lieber-DeCarli ethanol diet or pair-fed control diet for 4 weeks. Kupffer cells were isolated and nucleofected with 25 nM of scrambled siRNA or siRNA targeted to (A) TLR4, (B) TLR2, (C) RHAMM and (D) CD44. 24 h post-nucleofection, expression of (A) TLR4 (green), (B) TLR2 (red), (C) RHAMM (red) and (D) CD44 (red) was visualized by immunostaining with specific antibodies. Nuclei were labelled with DAPI (blue). All images were acquired using 20x objectives and are representative of 3 independent experiments.

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