Fig. 5
From: A novel in-vitro expression assay by LC/MS/MS enables multi-antigen mRNA vaccine characterization
Stability Study of Quadrivalent Flu LNPs: Relationship between %Antigen/HEK, % Antigen Positive Cells and MFI. The mRNA vaccine stability was measured by IVE-LC/MS/MS and IVE-flow cytometry. HEK293T cells were dosed with a quadrivalent mRNA vaccine. Overlays of the change in expressed antigen by IVE-LC/MS/MS and the percentage of antigen positive cells and MFI ratio by flow cytometry for up to 12 months at -80 °C (Panels A-D) and at 5 °C (Panels E–H) for four HA antigens. Each data point measured by IVE-LC/MS/MS is representing the average biological replicates (n = 3, %RSD ≤ 6%) and each IVE-Flow cytometry is representing the average of 2 biological replicates.
